Prequel to the first From Dusk Till Dawn is set in Mexico in the early 1900s which begins with the escape of Johnny Madrid, a dangerous local outlaw, from the gallows who then kidnaps his hangmans beautiful daughter, Esmeralda, with a little help from Reece, a female outlaw from the U.S. With the hangman and a local posse on their trail, Johnny meets with his gang who all rob a stagecoach which contains American author Ambrose Bierce along with newlywed couple John and Mary Newlie. As night falls, all parties coincidently seek shelter in an isolated inn/whorehouse which is run by vampires led by the high priestess Quixtla who targets Esmeralda. Esmeralda is revealed to be the half-human, half-vampire princess Santanico Pandemonium, whom the vampires want as their heir in which all the humans must join forces if they are to survive the night from the vicious blood-suckers.